小鼠增殖細(xì)胞核抗原(PCNA)ELISA試劑盒子科生物免費(fèi)代測,2016年年中*!進(jìn)行詢價,全場可以享受7折優(yōu)惠!并可享受滿減活動,滿10000減1000,滿5000減200元,滿1000元減50元,期待與您的合作!
小鼠增殖細(xì)胞核抗原(PCNA)ELISA試劑盒●品牌:子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
●產(chǎn)品說明書:可添加在線客服索要原版詳細(xì)操作說明書,或者留下您的,我們會將產(chǎn)品詳細(xì)說明書發(fā)送至您的
●價格:電議,或咨詢在線客服,或者以郵件形式發(fā)到我司zikerbio進(jìn)行詢價,我們將在*時間回復(fù)您!深圳子科生物提供大量品牌進(jìn)口原裝,分裝,以及穩(wěn)定性強(qiáng)的國產(chǎn)ELISA試劑盒,性價比*。咨詢!
小鼠增殖細(xì)胞核抗原(PCNA)ELISA試劑盒檢測方法:雙抗體夾心法、雙抗原夾心法、間接法測抗體、雙位點一步法、 競爭法 、捕獲法、ABS-ELISA方法,我們子科生物主要采用雙抗體夾心法。詳細(xì)可以咨詢我們銷售人員。ELISA方法測定可用的標(biāo)本十分廣泛,體液(如血清)、分泌物(唾液)和排泄物(如尿液、糞便)等均可作標(biāo)本以測定其中某種抗體或抗原成份。有些標(biāo)本可直接進(jìn)行測定(如血清、尿液),有些則需經(jīng)預(yù)處理(如糞便和某些分泌物)。大部分ELISA檢測均以血清為標(biāo)本。血漿中除尚含有纖維蛋白原和抗凝劑外,其他成份均同等于血清。制備血漿標(biāo)本需借助于抗凝劑,而血清標(biāo)本只要待血清自然凝固、血收縮后即可取得。除殊情況外,在臨床研究檢驗中均以血清作為檢測標(biāo)本。在ELISA實驗中血漿和血清可同等應(yīng)用。
客戶購買子科生物須知:
1、子科生物所提供的試劑產(chǎn)品,均為科研試劑,僅供科研使用,不得用于注射、藥品、食用,血更不能用于臨床檢測和動物防疫檢測或其他用途,對此造成的任何利潤與損失、間接損失、損害承擔(dān)責(zé)任經(jīng)濟(jì)糾紛,我司不承擔(dān)任何責(zé)任。
2、買方在提貨或收貨時,請仔細(xì)檢查貨物包裝,如發(fā)現(xiàn)包裝破損,應(yīng)拒收或拍照取證,隨后轉(zhuǎn)發(fā)給我司,買方一旦簽收,我司將不對產(chǎn)品的包裝狀態(tài)負(fù)責(zé);買方收到貨品后,應(yīng)及時清點,若發(fā)現(xiàn)貨物短缺,應(yīng)于簽收當(dāng)天通知我司,若簽收次日我司尚未收到買方通知,則視為買方確認(rèn)無短缺。
3、我們向您保證我們提供的產(chǎn)品均經(jīng)過鑒定為合格產(chǎn)品,如果真的有任何質(zhì)量問題,請您在收到產(chǎn)品1個月內(nèi)以書面的形式及時通知我司,超過時間不予受理,我們將給您提供*周到及時的技術(shù)售后處理。
操作步驟
1、 取出試劑盒,于室溫(20-25℃)放置15-30分鐘。實驗過程應(yīng)在室溫(20-25℃)內(nèi)進(jìn)行。
2、 取出酶標(biāo)板,按照標(biāo)準(zhǔn)品的次序分別加入50μl的標(biāo)準(zhǔn)品溶液于空白微孔中。
3、空白微孔中加入50μl的樣品,空白對照加入50μl的蒸餾水;
4、 在樣品孔中加入10μl的生物素;(不含空白對照孔,切忌:生物素只加樣品孔!)
5、在各孔中加入100μl的酶標(biāo)記溶液;(不含空白對照孔)
6、將酶標(biāo)板用封口膠密封后,37℃孵育反應(yīng) 1小時;(在孵育箱中保持穩(wěn)定的溫度與濕度)
7、 充分清洗酶標(biāo)板3-5次,保持各孔有充足的水壓;(濃縮洗滌液以1:100的比例與蒸餾水稀釋)
8、酶標(biāo)板洗滌后用吸水紙*拍干;
9、各孔加入顯色劑A、B液各50μl;(不含空白對照孔)
10、20-25℃下避光反應(yīng)15分鐘;
11、各孔加入50μl終止液,終止反應(yīng);
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